• The BB team is looking for employees, who are ready to work with mephedrone, amphetamine, methamphetamine and other substances.
    High wage! Stable job.

    Requirements:

    • Make a minimum deposit of 50 EUR
    • Receive a batch of goods (from 25 g and more, depending on the deposit)
    • Pack the goods in bags (1-2g) and hide them in safe places in your city.

    We going to do the rest work! As soon as the goods are sold, you'll get money for each successfully sold package!

    Relevant for all EU countries and UK

    Contact : @Madre

THC products testing protocol

G.Patton

Expert
Joined
Jul 5, 2021
Messages
2,387
Solutions
3
Reaction score
2,327
Points
113
Deals
1

Introduction.

You bought THC containing product with unusual effects, or you think this product contain admixtured substances, you want to figure out pollutants and admixtures with help of testing experiments before use this stuff. Open this article and use it as a guide for experimenting. The list of manipulations with THC containing products, a brief description of the products, and useful information for home tests are presented below.
IKLZpg6TC3

Forms.

Cannabis (also known as marijuana, weed, pot, grass, hemp, and many others) is a preparation of the cannabis plant that produces psychoactive effects when consumed (via smoking, vaporizing, or ingestion). The principal psychoactive constituent of cannabis is tetrahydrocannabinol (THC), which makes up one of 483 known compounds in the plant, including at least 84 other cannabinoids such as cannabidiol (CBD), cannabinol (CBN), tetrahydrocannabivarin (THCV), and cannabigerol (CBG). At least three species are recognized: Cannabis sativa, Cannabis indica, and Cannabis ruderalis.

Cannabis is consumed in many ways, all of which involve heating to decarboxylate THCA in the plant into THC.
  • Smoking, which typically involves burning and inhaling vaporized cannabinoids ("smoke") from small pipes, bongs (portable versions of hookahs with a water chamber), paper-wrapped joints or tobacco-leaf-wrapped blunts, and other items.
  • Vaporizer, which heats any form of cannabis to 165–190 °C (329–374 °F), causing the active ingredients to evaporate into vapor without burning the plant material (the boiling point of THC is 157 °C (315 °F) at atmospheric pressure).
  • Cannabis tea, which contains relatively small concentrations of THC because THC is an oil (lipophilic) and is only slightly water-soluble (with a solubility of 2.8 mg per liter). Cannabis tea is made by first adding a saturated fat to hot water (e.g. cream or any milk except skim) with a small amount of cannabis.
  • Edibles, where cannabis is added as an ingredient to one of a variety of foods, including butter and baked goods. In India, it is commonly made into a beverage, bhang.
  • Capsules, typically containing cannabis oil, and other dietary supplement products, of which some 220 were approved in Canada in 2018.
HijaST2buE
V7mYiaoM3L
ZwPaDTnQMo
RqV50wYBDC
1UWlmitRdh
J8P6msRZSF
GzSlPRQUXL

Possible adulterant substances.

Marijuana or other THC containing products are substituted or admixtured quite rarely due to widespread, low producing cost and moderate laws to cannabis products. However, sometimes impregnated by synthetic cannabinoids are met in streets. Synthetic cannabinoids encompass a variety of distinct chemical classes: the classical cannabinoids structurally related to THC; the nonclassical cannabinoids (cannabimimetics), including the aminoalkylindoles, 1,5-diarylpyrazoles, quinolines, and arylsulphonamides; and eicosanoids related to the endocannabinoids. Usually, meet MDMB-4en-PINACA (5Cl-ADB-A), JWH row substances, ADB-BUTINACA, 4F-MDMB-BINACA, ADB-FUBINACA, 4F-MDMB-BICA, ADB-BINACA and so on. Also, cannabis products contain another phytocannabinoids, such as CBD, CBN, CBG, CBC etc. Here you can see a list of analysis reports of US drug testing laboratory.

Phytocannabinoids can be defined as any plant-derived natural product capable of either directly interacting with cannabinoid receptors or sharing chemical similarity with cannabinoids, or both. The entourage effect is a proposed mechanism, by which compounds present in cannabis, which are largely non-psychoactive by themselves modulate the overall psychoactive effects of the plant (these resulting principally from the action of the main psychoactive component of cannabis, tetrahydrocannabinol (THC)).
XRm9Cfh5DZ
Algorithm of procedures:

1. First, carry on Duquenois–Levine color experiment for defining THC and cannabinoids in your product. Also, you have to provide experiment with Fast Blue B Salt, as described below. You may carry out one of them optionally.

You need:
  • Filter paper;
  • A: petroleum ether;
  • B: Fast Blue B Salt 1% diluted with anhydrous sodium sulfate;
  • C: sodium bicarbonate 10% aq. solution.
Fold two pieces of filter paper folded together in four and fold back the edges slightly so that a funnel forms, place a small amount of ground sample in the center of the top layer of paper. Add two drops of reagent A and allow the liquid to penetrate into the bottom layer of filter paper. Throw away the top sheet of paper, let the bottom sheet dry. Apply a small amount of reagent B to the center of the filter paper, and add two drops of reagent C.

Results.
A purple-red spot in the center of the filter paper indicates a cannabis product.
This color results from a mixture of colors typical of cannabinoids, which are the main components of cannabis: THC = red, CBN = purple, CBD = orange.

2. Extraction from smoking mixtures
This step is needed to carry on for extracting psychoactive substances from THC containing products before testing them by following methods. The multiple functional groups associated with synthetic cannabinoids necessitate a generalized extraction approach. For this analysis, an acid/base combined extraction followed by centrifugation was employed. It is also possible to perform the extraction using methanol incubation. Either approach will extract substantial amounts of matrix components. Using the acid/base approach, an aliquot of homogenized sample (50 – 100 mg) was acidified by adding 1 ml of deionized water, followed by three drops of 10% hydrochloric acid. Next, 1 ml of solvent (95% methylene chloride/5% isopropanol v/v) was added and the sample mixed. The sample was then centrifuged, and the bottom solvent layer retained and set aside. Two drops of concentrated ammonium hydroxide and 1 ml of the solvent (95% methylene chloride/5% isopropanol v/v) were added to the remaining aqueous mixture (top layer). The sample was mixed and centrifuged again. The bottom solvent layer was removed, combined with the first bottom solvent layer collected, and then mixed briefly. Solvent have to be evaporated. Received substance ready for testing.

3. Thirdly, check your extracted product with the declared JWH by LF tests (drug testing kits). You will receive clear result about presence in your stuff THC and synthetic cannabinoids. In addition, you may check presence of other substances by multiple drug testing kit. K2/ Spice test reveal JWH-018, JWH-073, JWH-210, JWH-398, MAM2201 and so on.

4. You did not find any pollutants, and you still doubt in your product or LF tests reveal admixtured narcotic substance, lead experiments with test reagents. Use «Drugs testing reagents». These methods help to determine kind of admixtures. There are manuals, which are described checking method procedures and meaning, where you can find methods of reagent synthesis. With help of drug testing reagents, you may determine the kind of your adulterants. Liebermann and Froehde test reagents are primary tests used for synthetic cannabinoid row determination. According to data from testing reagent experiments, you may compare and approve result by TLC.
GP8RpwLEBh
For TLC, you can use one of the following solvent systems:
  • A: petroleum ether 60/90:diethyl ether 4:1.
  • B: cyclohexane:diisopropyl ether:diethylamine 52:40:8.
  • C (for cannabinoidic acids): n-hexane:dioxane:methanol 7:2:1.

TLC results:
The RfX100 values may vary depending on the laboratory conditions (temperature, humidity etc.) and other parameters (e.g. age and quality of the cannabis product tested). It is therefore considered good practice to analyze a sample together with cannabinoid control samples on the same TLC plate.
E4igLhjyBk

Conclusion.

The best ways to determine composition of THC containing product are GC-MS or LC-MS analysis because this stuff may contain wide number of natural substances (phytocannabinoids, flavonoids, terpenes) analogues, which may cause hard to interpret test results. However, this manual allows to identifying some of them and approve results by different methods.
 
Last edited:
  • Free product samples

    Testing products from new vendors and manufacturers.

    Get free samples for testing now!

  • Always stay in touch with BB forum. Element/Matrix.

    Connect notifications to always stay in touch with the forum!

    Connect

  • The BB Forum team is looking for cooperation:

    • Traffic arbitrage specialists
    • Spammers
    • Advertising agencies
    • Bloggers/Vloggers
    • TOR sites directories
    • Creative people who can create viral content
    • Administrators of Telegram Channels and Groups

      We will pay more for your traffic than our competitors! $0.1 per visitor!!!If you are interested in, write to the administrator.
Top