Extraction of Piperine from Black Pepper by Direct Method without Using Soxhlet Extractor

[GhostChemist]

Extraction with Soxhlet Extractor
http://bbzzzsvqcrqtki6umym6itiixfhni37ybtt7mkbjyxn2pgllzxf2qgyd.onion/media/piperine-extraction.113/

Structure of piperin is present on Scheme 1

Scheme 1​

Reagents and materials:​

• Ethanol 88% 600-700 ml​
• Powder black pepper 300 g​
• 24 g Potassium hydroxide KOH​
• 1600 ml distilled water​
• Paper filters diameter 180 mm​
• Flask 2000 ml​
• Beakers​
• Funnel​
• Heater​

Place 300 g of black pepper powder and 500 ml of 88% EtOH into a 2000 ml flask. Fig 1​

Fig 1​

The mixture is boiled under a reflux condenser for 5-7 hours. Fig 2​

Fig 2​

A solution of potassium hydroxide is prepared: 24 g Potassium hydroxide KOH + 200 ml water. Fig 3​

Fig 3​

The piperine extract in ethanol is filtered warm (40-50℃) into the prepared potassium hydroxide solution (if there is a deficiency of ethanol, more than half of the solvent can be distilled off and saved before mixing with the potassium hydroxide solution). The addition of potassium hydroxide or another alkali is necessary to remove resins. Fig 4​

Fig 4​

Powder of pepper is additionally washed with 100-200 ml of warm (40℃) ethanol on a filter. Fig 5​

Fig 5​

The resulting filtered solution is heated to 60-70℃. Fig 6​

Fig 6​

The heated solution is transferred to a large 2000 ml beaker. Fig 7​

Fig 7​

Water is added to the solution until sediment formation stopped (~1400 ml water). Fig 8​

Fig 8​

The resulting suspension is cooled. Fig 9​

Fig 9​

The piperine precipitate is filtered on filters. Piperine is much dispersed and passes through the pores of most filter materials. Fig 10​

Fig 10​

The piperine precipitate is washed with water until a neutral reaction is achieved (it is necessary to remove the alkali). Fig 11​

Fig 11​

Piperine is dried in a desiccator without access to air and sunlight. Fig 12​

Fig 12​

Yield is 7.8 g​

Fig 13​